News
人材検索について
ハイパフォーマンススポーツに係る研究者やサポートスタッフ等の人材情報の検索ができます。HPSCに現在勤務する職員（HPSCスタッフ）、HPSCの勤務経験者（HPSC OB/OG）、HPSCの各種事業における協力者（HPSC事業協力者）のカテゴリーに分けております。人材データベースへのご登録はHPSCからご依頼させて頂いた方のみとしております。
最終更新日：2024/02/02
枝 伸彦
エダ ノブヒコ
(Nobuhiko Eda)
論文
- タイトル
- タイトル（英）
- Development of simple detection method of polyreactive secretory immunoglobulin A in saliva
- 参照URL
- https://researchmap.jp/7000005037/published_papers/38562159
- 著者
- 著者（英）
- Ziyue Sun,Yuto Inai,Kyoko Koseki,Satoshi Oikawa,Nobuhiko Eda,Takao Akama
- 担当区分
- 概要
- 概要（英）
- The level of secretory immunoglobulin A (SIgA) in saliva is associated with the risk of upper respiratory infection and has been identified as a condition indicator for athletes and an indicator of mucosal immune function in the elderly. In addition, it has been suggested that non-specific polyreactive SIgA is present in human saliva and colostrum. The purpose of this study was to establish a new detection method of polyreactive salivary SIgA. Using saliva samples from eight healthy individuals, indirect ELISA was used to detect anti-LPS antibodies (LPS ELISA), and competitive ELISA was used to detect LPS antibodies after competition with DNA (LPS-DNA ELISA). The difference in SIgA concentration between LPS ELISA and LPS-DNA ELISA is thought to be the concentration of the polyreactive SIgA, which responds to both DNA and LPS. First, we determined the concentration for coating LPS and DNA as 10 μg/ml, and the saliva sample dilution was determined as 1/2. The concentration of anti-LPS antibody contained in the saliva sample, which is strongly reactive to LPS, was defined as the standard. Compared to the SIgA concentration determined by LPS ELISA, SIgA concentration of LPS-DNA ELISA did not decrease in two samples, but was reduced in 6 samples. The decrease in SIgA concentration is thought to be the concentration of polyreactive SIgA that reacts with both DNA and LPS. Notably, we found sample-to-sample differences in the concentration of polyreactive SIgA.
- 出版者・発行元
- 出版者・発行元（英）
- The Japanese Society of Physical Fitness and Sports Medicine
- 誌名
- 誌名（英）
- The Journal of Physical Fitness and Sports Medicine
- 巻
- 11
- 号
- 4
- 開始ページ
- 207
- 終了ページ
- 212
- 出版年月
- 2022年7月25日
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- 2186-8131
- DOI URL
- https://doi.org/10.7600/jpfsm.11.207
- 共同研究・競争的資金等の研究課題