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最終更新日：2024/05/03
玉井 伸典
タマイ シンスケ
(Shinsuke TAMAI)
論文
- タイトル
- タイトル（英）
- Establishing a Sequencing Method for the Whole Mitochondrial DNA of Domestic Dogs
- 参照URL
- https://researchmap.jp/shinsuke-tamai/published_papers/42852209
- 著者
- 著者（英）
- Takehito Sugasawa,Yuki Matsumoto,Hui Fang,Tohru Takemasa,Ritsuko Komine,Shinsuke Tamai,Wenchao Gu,Kei Tanaka,Yasuharu Kanki,Yoichiro Takahashi
- 担当区分
- 概要
- 概要（英）
- In human beings, whole mitochondrial DNA (mtDNA) sequencing has been widely used in many research fields, including medicine, forensics, and genetics. With respect to the domestic dog (Canis lupus familiaris), which is commonly recognized as being an additional member of the traditional human family structure, research studies on mtDNA should be developed to expand and improve our collective knowledge of dog medicine and welfare as it seems that there is still room for further development in these areas. Moreover, a simple and robust method for sequencing whole mtDNA that can be applied to various dog breeds has not yet been described in the literature. In the present study, we aim to establish such a method for the whole mtDNA sequencing of the domestic dog. In the experiments we conducted, oral mucosa DNA samples obtained from six Japanese domestic dogs were used as a template. We designed four primer pairs that could amplify approximately 5 kbp from each region of the mtDNA and validated several PCR conditions. Subsequently, the PCR amplicons were pooled and subjected to library preparation. The sequencing of the libraries was performed using next-generation sequencing (NGS), followed by bioinformatics analysis. Our results demonstrate that the proposed method can be used to perform highly accurate resequencing. We believe that this method may be useful for future research conducted to better understand dog medicine and welfare.
- 出版者・発行元
- 出版者・発行元（英）
- MDPI AG
- 誌名
- 誌名（英）
- Animals
- 巻
- 13
- 号
- 14
- 開始ページ
- 2332
- 終了ページ
- 2332
- 出版年月
- 2023年7月17日
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.3390/ani13142332
- 共同研究・競争的資金等の研究課題