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最終更新日：2026/04/15
門口 智泰
(Tomoyasu Kadoguchi)
論文
- タイトル
- タイトル（英）
- Analysis of proteins released from osteoarthritic cartilage by compressive loading.
- 参照URL
- https://researchmap.jp/m-s-m-i/published_papers/44475692
- 著者
- 著者（英）
- Hirotaka Tsuno,Nobuho Tanaka,Masashi Naito,Satoru Ohashi,Mitsuyasu Iwasawa,Tomoyasu Kadoguchi,Hiroyuki Mitomi,Toshihiro Matsui,Hiroshi Furukawa,Naoshi Fukui
- 担当区分
- 概要
- 概要（英）
- In osteoarthritis (OA), synovial pathology may be induced by proteins released from degenerated cartilage. This study was conducted to identify the proteins released from OA cartilage. OA cartilage was obtained from OA knees at macroscopically preserved areas (PRES) and degenerated areas (DEG), while control cartilage (CONT) was collected from non-arthritic knees. Released proteins were obtained from these cartilage samples by repeatedly applying compressive loading, which simulated loading on cartilage in vivo. The released proteins were analyzed comprehensively by antibody array analyses and a quantitative proteomic analysis. For several proteins, the exact amounts released were determined by Luminex assays. The amount of active TGF-β that was released was determined by an assay using genetically-engineered HEK cells. The results of the antibody array and proteomic analyses revealed that various biologically active proteins are released from OA cartilage, particularly from DEG, by loading. The Luminex assay confirmed that several alarmins, complement proteins C3a and C5a, and several angiogenic proteins including FGF-1, FGF-2 and VEGF-A were released in greater amounts from DEG than from CONT. The HEK cell assay indicated that active TGF-β was released from DEG at biologically significant levels. These findings may be helpful in understanding the pathology of OA.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- Scientific reports
- 巻
- 13
- 号
- 1
- 開始ページ
- 18292
- 終了ページ
- 18292
- 出版年月
- 2023年10月25日
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1038/s41598-023-45472-x
- 共同研究・競争的資金等の研究課題