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最終更新日：2025/09/02
曽根 良太
ソネ リョウタ
(Ryota Sone)
論文
- タイトル
- タイトル（英）
- Evaluation of Saliva Collection and DNA Extraction Methods for Practical Application of Salivary Human Herpesvirus 6 and 7 Assays
- 参照URL
- https://researchmap.jp/rsone/published_papers/49446009
- 著者
- 著者（英）
- Shinsuke Tamai,Ryota Sone,Koichi Watanabe,Kazuhiro Shimizu
- 担当区分
- 概要
- 概要（英）
- Salivary human herpesvirus 6 and/or 7 (HHV-6/7) have recently attracted attention as microbiological markers of physiological fatigue in laborers and athletes. However, the accuracy and efficiency of the HHV-6/7 assays can be improved for practical application. We conducted three experiments to identify suitable saliva collection and DNA extraction methods for practical salivary HHV-6/7 assays. The main experiment compared the data, including template DNA or HHV-6/7 concentrations, among three saliva collection methods (cotton, synthetic, and no swabs) and two DNA extraction methods (magnetic bead-based and silica column-based). It showed that using swabs had adverse effects: lower template DNA concentration, lower HHV-6/7 detection rates, higher coefficient of variation values, and lower concentrations. Moreover, magnetic bead-based methods resulted in higher HHV-6/7 detection rates and lower coefficient of variation values. Sub-experiment 1 examined practical saliva collection methods and demonstrated that the stimulated spitting method could collect saliva in a shorter time with lower subjective stress than the unstimulated spitting and stimulated swabbing methods. Sub-experiment 2 investigated diurnal variation in salivary HHV-6/7 levels but did not show diurnal variation. These findings suggest that (1) the combination of stimulated spitting saliva collection and magnetic bead-based DNA extraction is most suitable for practical salivary HHV-6/7 assays, and (2) saliva collection can be conducted whenever needed.
- 出版者・発行元
- 出版者・発行元（英）
- MDPI AG
- 誌名
- 誌名（英）
- Viruses
- 巻
- 17
- 号
- 3
- 開始ページ
- 411
- 終了ページ
- 411
- 出版年月
- 2025年3月
- 査読の有無
- 査読有り
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.3390/v17030411
- 共同研究・競争的資金等の研究課題