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最終更新日：2024/07/24
高橋 祐美子
(Yumiko TAKAHASHI)
論文
- タイトル
- タイトル（英）
- Branched-chain amino acid supplementation suppresses the detraining-induced reduction of mitochondrial content in mouse skeletal muscle.
- 参照URL
- https://researchmap.jp/yumikotakahashi/published_papers/40729597
- 著者
- 著者（英）
- Yutaka Matsunaga,Yuki Tamura,Kenya Takahashi,Yu Kitaoka,Yumiko Takahashi,Daisuke Hoshino,Tomoyasu Kadoguchi,Hideo Hatta
- 担当区分
- 概要
- 概要（英）
- Exercise training enhances oxidative capacity whereas detraining reduces mitochondrial content in skeletal muscle. The strategy to suppress the detraining-induced reduction of mitochondrial content has not been fully elucidated. As previous studies reported that branched-chain amino acid (BCAA) ingestion increased mitochondrial content in skeletal muscle, we evaluated whether BCAA supplementation could suppress the detraining-induced reduction of mitochondrial content. Six-week-old male Institute of Cancer Research (ICR) mice were randomly divided into four groups as follows: control (Con), endurance training (Tr), detraining (DeTr), and detraining with BCAA supplementation (DeTr + BCAA). Mice in Tr, DeTr, and DeTr + BCAA performed treadmill running exercises [20-30 m/min, 60 min, 5 times/week, 4 weeks]. Then, mice in DeTr and DeTr + BCAA were administered with water or BCAA [0.6 mg/g of body weight, twice daily] for 2 weeks of detraining. In whole skeletal muscle, mitochondrial enzyme activities and protein content were decreased after 2 weeks of detraining, but the reduction was suppressed by BCAA supplementation. Peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) protein content, a master regulator of mitochondrial biogenesis, was decreased by detraining irrespective of BCAA ingestion. Regarding mitochondrial degradation, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3), a mitophagy-related protein, was significantly higher in the Tr group than in the DeTr + BCAA group, but not different from in the DeTr group. With respect to mitochondrial quality, BCAA ingestion did not affect oxygen consumption rate (OCR) and reactive oxygen species (ROS) production in isolated mitochondria. Our findings suggest that BCAA ingestion suppresses the detraining-induced reduction of mitochondrial content partly through inhibiting mitophagy.
- 出版者・発行元
- 出版者・発行元（英）
- 誌名
- 誌名（英）
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology
- 巻
- 36
- 号
- 12
- 開始ページ
- e22628
- 終了ページ
- 出版年月
- 2022年12月
- 査読の有無
- 招待の有無
- 掲載種別
- 研究論文（学術雑誌）
- ISSN
- DOI URL
- https://doi.org/10.1096/fj.202200588R
- 共同研究・競争的資金等の研究課題